A chemically defined medium containing nicotinamide adenine dinucleotide (NAD) supported the growth of Actinobacillus pleuropneumoniae biotype 2 (Bertschinger 2008/76), whereas the same medium lacking NAD did not. Nicotinamide mononucleotide, nicotinamide riboside, and nicotinamide could substitute for NAD, whereas nicotinic acid adenine dinucleotide, nicotinic acid mononucleotide, nicotinic acid, and quinolinic acid could not. Hence, although described as being V factor independent, this organism can exhibit V-factor-dependent growth.
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Niven et al. (1988) studied this question.
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