Increased UCP1 expression in the perirenal adipose tissue of patients with renal cell carcinoma

Figures

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  Figure 1.. Characterization of different parts of the PAT. (A) Morphology of the upper and lower renal poles. Representative hematoxylin and eosin (H&E) staining images are shown (n=20). (B) Size of adipocytes in the upper and lower renal pole fat pads (magnification, ×200). Data represent the mean ± standard deviation (SD), n=20. (C) Number of adipocytes in the upper and lower renal pole fat pads (magnification, ×200). Data represent the mean ± SD, n=20. (D) UCP1 staining in the upper and lower renal poles. Representative images are shown (n=20). (E) Quantification of UCP1 staining in the upper and lower renal poles. Data represent the mean ± SD, n=10. (F and G) Western blot analysis of the UCP1 level in the upper and lower renal poles (n=6). (H) RT-qPCR analysis of the mRNA levels of UCP1 and other BAT genes in the upper and lower renal poles (n=104). (I and J) RT-qPCR analysis of the mRNA levels of white adipocyte and brown adipocyte genes in the upper and lower renal poles (n=104). (K-M) RT-qPCR analysis of the mRNA levels of metabolism-related genes and mitochondrial genes in the upper and lower renal poles (n=104). PAT, perirenal adipose tissue; RT-qPCR, reverse…

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  Figure 2.. Expression of UCP1 and other genes in the bSAT of the RCC and control groups. (A) RT-qPCR analysis of UCP1 and other BAT genes in bSAT (n=104). (B) RT-qPCR analysis of brown adipocyte genes in the RCC and control groups (n=104). (C) RT-qPCR analysis of white adipocyte genes in the RCC and control groups (n=104). (D-F) RT-qPCR analysis of the mRNA levels of metabolism-related genes and mitochondrial genes in the RCC and control groups (n=104). RCC, renal cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; UCP1, uncoupling protein 1; BAT, brown adipose tissue; bSAT, back subcutaneous adipose tissue.

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  Figure 3.. Higher UCP1 expression in the PAT of the RCC group. (A) Morphology of the PAT. Representative hematoxylin and eosin (H&E) staining images are shown (n=20). (B) Size of adipocytes in the RCC and control groups (magnification, ×200). Data are presented as the mean ± standard deviation (SD), n=20 (***P<0.001). (C) Number of adipocytes in the RCC and control groups (magnification, ×200). Data are presented as the mean ± SD, n=20. (***P<0.001). (D) UCP1 staining in the PAT of the RCC and control groups. Representative images are shown (n=20). (E) Quantification of UCP1 staining in the RCC and control groups. Data are presented as the mean ± SD, n=10 (***P<0.001). (F and G) Western blot analysis of the UCP1 level in the PAT of the RCC and control groups (n=8) (***P<0.001). (H) RT-qPCR analysis of the mRNA levels of UCP1 and other BAT genes in the RCC and control groups (**P<0.01) (n=104). (I and J) RT-qPCR analysis of the mRNA levels of white adipocyte and brown adipocyte genes in the PAT of the RCC and control groups (n=104) (*P<0.05, ***P<0.001). (K-M) RT-qPCR analysis of the mRNA levels of adipocyte metabolism-related genes and mitochondrial function genes in the PAT of the RCC and…

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  Figure 4.. Association between UCP1 mRNA expression and Fuhrman grade. There was an increase in UCP1 mRNA expression with advancing Fuhrman grade. r is the Spearman correlation coefficient and P<0.05 indicates the significance level (n=50). UCP1, uncoupling protein 1.

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  Figure 5.. Association between UCP1 mRNA expression and T stage. There was an increase in UCP1 mRNA expression with advancing T stage. r is the Spearman correlation coefficient and P<0.05 indicates the significance level (n=50). UCP1, uncoupling protein 1.