Anti-inflammatory and antinociceptive activity of field-growth plants and tissue culture of Cleome spinosa (Jacq.) in mice

Methanol extracts (ME) of Cleome spinosa obtained from plants of natural growing areas (NP) and acclimatized plants (AP), as well as from the lipophilic mass containing beta-carotene (LMBC) produced from callus cultures were evaluated for their anti-inflammatory and antinociceptive potentials. These properties were studied using carrageenan-induced paw edema and acetic acid-induced writhing animal models. The ME from leaves of NP and AP 10 mg/kg, intraperitoneally (i.p.) caused a significant inhibition of carrageenan-induced paw edema. Antinociceptive activity was observed in extracts from leaves of AP, leaves and stems of NP and LMBC, at 50 mg/kg (i.p.), corresponding to half concentration of the standard drug dipyrone (100 mg/kg). The extracts did not present toxicity as determined by Artemia salina bioassay. Our results demonstrated that C. spinosa has anti-inflammatory and antinociceptive properties, confirming its popular use, and also served to extend the use of plant biotechnology tools for investigations on the pharmacological potential and phytochemical exploitation of this species.