Answers are grounded in this paper and cite their sources.
Change media to fresh fibroblast media, *See steps 8-9 Spin at 800xg for 1 hour at RT *See steps 5-7 Incubate plate at 37°C overnight Repeat steps 5-9 Aspirate media and add 2 ml of 4F Retro virus + Polybrene Passage transduced fibroblasts from 6w plate to a 10 cm of MEFS *See step 12 Feed everyday with WiCell hES media.
No takes yet. Share an insight, caveat, or question.
Travis W. Berggren (2014) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: