In Vitro Antifungal Susceptibility Testing of 5 Antifungal Agents against Dermatophytic Species by CLSI (M38-A) Micro Dilution Method

Cases of dermatophytoses have increased over the past few decades.In the last few years, a number of newer less toxic antifungal drugs have become available for clinical use.The increased use of antifungal, often for prolonged periods, has led to the recognition of the phenomenon of acquired antifungal resistance among previously susceptible strains or species and to the increased incidence of infections with less common species.Our study mainly focused on the in vitro susceptibility of clinical isolates of dermatophytes against frequently used 5 antifungal agents.The microbroth dilution method was performed according to CLSI standards.In the present study antifungal susceptibility testing was done by micro dilution method of dermatophytes against 5 antifungal agents namely, ketoconazole (imidazoles) fluconazole, itraconazole (triazoles), griseofulvin and terbinafine and their activity against significant number of strains, representing a wide spectrum of dermatophyte species is assessed.