Key result
The newly developed real-time RT-PCR assays demonstrated high sensitivity and specificity for distinguishing Cluster IV H3N2v from seasonal H3N2 influenza viruses without cross-reactivity.
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From 2005 to July 6, 2018, a total of 435 swine-origin influenza A H3N2 variant virus (H3N2v) infections in humans were reported in the USA. The largest H3N2v outbreak in the USA occurred in 2011-2012. This virus obtained the HA gene from the human seasonal H3N2 influenza A viruses (seasonal H3N2) via human-to-swine transmission in the mid-1990s and was classified as Cluster IV H3N2v. For early detection of public health threats associated with Cluster IV H3N2v distinct from seasonal H3N2, we developed highly specific and sensitive one-step real-time RT-PCR assays directly targeting the HA genes of Cluster IV H3N2v and seasonal H3N2. These assays are useful for the systematic surveillance and identification of Cluster IV H3N2v.
New real-time RT-PCR assays allow for highly specific and sensitive detection of the Cluster IV H3N2v influenza virus.
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Saito et al. (2018) studied Influenza A H3N2 variant virus (H3N2v) and seasonal H3N2 (n=18). Real-time RT-PCR assays for Cluster IV H3N2v and seasonal H3N2 vs. TypeA rRT-PCR assay was evaluated on Detection limit and specificity of the rRT-PCR assays. The newly developed real-time RT-PCR assays demonstrated high sensitivity and specificity for distinguishing Cluster IV H3N2v from seasonal H3N2 influenza viruses without cross-reactivity.
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