Logic-Based Dual-Functional DNA Tweezers with Protein and Small Molecule as Mechanical Activators

In the present work, dual-functional DNA tweezers were constructed for the multiplex detection of thrombin and adenosine triphosphate (ATP). The aptamers for thrombin and ATP were respectively immobilized on magnetic beads (MBs). Then they hybridized with complementary sequences at the ends of opening strands C in and D in . In the presence of thrombin and ATP, C in and D in were released as the results of competition. The tweezers contained two symmetrical components, and six DNA strands were hybridized to form the closed state. When the opening strands were introduced, the tweezers were driven to the open state, releasing two inert double-stranded waste products.