Key result
DOCA-salt treatment markedly increases adrenal ANP mRNA and peptide expression in rats.
Why the study?
The role and regulation of adrenal atrial natriuretic peptide (ANP) synthesis in body fluid homeostasis under deoxycorticosterone acetate-salt treatment was not fully understood.
Hypothesis-generating for adrenal ANP in mineralocorticoid models; leaves open human translation and clinical relevance.
Our previous study demonstrated that human adrenal medulla is a site of atrial natriuretic peptide (ANP) synthesis. To further evaluate the role of adrenal ANP in body fluid homeostasis, we investigated the changes in adrenal ANP in rats receiving deoxycorticosterone acetate (DOCA)-salt treatment. In situ hybridization and immunohistochemical study showed that adrenal ANP messenger RNA (mRNA) and ANP-like immunoreactivities (ANP-LI) were mainly localized in the zona glomerulosa and medulla of vehicle-treated rats. DOCA-salt treatment activated ANP mRNA and peptide expression in all adrenal zones, especially in the zona fasciculata/reticularis from 12 h to the entire 8-day study period. Using a semiquantitative RT-PCR technique, the relative quantities of ANP mRNA in the adrenals of the DOCA-salt-treated group were significantly increased from 1 to 8 days, whereas the adrenal weights of DOCA-salt-treated rats were significantly decreased from day 2 to day 8. Our results are the first to indicate that ANP is synthesized not only in the adrenal medulla but also in the adrenal cortex and their syntheses are markedly increased in DOCA-salt-treated rats. These results imply that adrenal ANP may participate in the intraadrenal regulation of adrenal function on water-electrolyte homeostasis in an autocrine or paracrine manner.
No takes yet. Share an insight, caveat, or question.
Feng‐Jie Lai (2000) studied Water-electrolyte homeostasis (DOCA-salt model). Deoxycorticosterone acetate (DOCA)-salt treatment vs. Vehicle was evaluated on Adrenal ANP mRNA and peptide expression. Deoxycorticosterone acetate (DOCA)-salt treatment significantly increased atrial natriuretic peptide (ANP) mRNA and peptide expression in the adrenal cortex and medulla of rats over 8 days.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: