Answers are grounded in this paper and cite their sources.
Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
No takes yet. Share an insight, caveat, or question.
Tuley et al. (2014) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: