Calsyntenin 3β Is Dynamically Regulated by Temperature in Murine Brown Adipose and Marks Human Multilocular Fat

Figures

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  Figure 1. CLSTN3β is enriched in human multilocular brown adipose tissue and correlates with UCP1 expression across fat depots. (A) Relative mRNA expression of CLSTN3β, CLSTN3α and S100b in human multilocular or unilocular perirenal brown fat and subcutaneous white fat. Data represent (fold change) means ± SEM. (B) Correlational analysis of all three genes vs. UCP1 across human fat depots (logarithmic values are plotted). Statistical differences are based on one-way-ANOVA with Kruskal Wallis post-test (A) or linear regression (B). Asterisks: **p < 0.01.

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  Figure 2. Clsnt3β is enriched in mouse brown fat and dynamically regulated by temperature in thermogenic adipose depots. (A) Relative gene expression of Clstn3β, Clsnt3α, S100b and Ucp1 in mouse BAT, SAT and VAT after chronic exposure to thermoneutrality (29°C, 21 days) and transition to cold (5°C) for 3, 7 or 21 days. Data are expressed relative to BAT at 29°C for each gene. (B) Brown fat fraction enrichment analysis showing gene expression in BAT stromal vascular fraction (SVF) or adipocytes (AD) of mice exposed chronically to thermoneutrality or cold (21 days). Data are expressed relative to adipocyte fraction at 29°C for each gene. (C) Regulation of gene expression in mouse BAT after chronic exposure to cold (5°C, 21 days) and transition to thermoneutrality (29°C) for 3 or 7 days. Data represent means ± SEM and are based on n = 3–6 mice per condition. Overall effects of temperature and depot are reported in graphs (two-way-ANOVA in A, B; one-way-ANOVA in C) and post-hoc comparisons between groups are indicated by asterisks. Asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.

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  Figure 3. Expression patterns of Clstn3β in mouse models of obesity. Gene expression of Clstn3β, Clsnt3α, S100b and Ucp1 in brown adipose tissue from lean, high-fat diet (HFD) fed or ob/ob mice (n = 11–12 mice per condition). Data represent means ± SEM, expressed relative to lean controls. One-way-ANOVA and post-hoc comparisons between groups are indicated in graphs. Asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.