HPLC–MS (electrospray ionization or atmospheric pressure chemical ionization) was applied to the analysis of cucurbitacins in medicinal plants. High molecular weight plant constituents were separated from cucurbitacins by gel chromatography of the crude methanolic extracts. HPLC was performed on a Zorbax SB-C-18 column using a 0.01% trifluoroacetic acid:acetonitrile gradient and diode array detection. The method described was sensitive, and its applicability was demonstrated through the determination of cucurbitacins in a number of medicinal plants such as Citrullus colocynthis (Cucurbitaceae), Bryonia cretica ssp. dioica (Cucurbitaceae), Gratiola officinalis (Scrophulariaceae), Picrorhiza kurroa (Scrophulariaceae) and Iberis umbellata (Brassicaceae).
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Sturm et al. (2000) studied this question.
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