Adaptation of an Insect Cell Line (Grace's Antheraea Cells) to Medium Free of Insect Hemolymph

This is the authors' abstract. We don't add key points for this paper.

Cultures of the insect cell line derived by Grace from Antheraea eucalypti Scott [Austrocaligula eucalypti (Scott)] were successfully adapted to medium supplemented with fetal bovine serum, whole-egg ultrafiltrate, and bovine plasma albumin instead of insect hemolymph. Cells, now in their 37th passage, have a population doubling time of 2.5 days; those of unadapted cultures, 4.2 days.