Key result
2′-N-(Pyren-1-yl)acetyl-2′-Amino-α-L-LNA probes successfully signaled the presence of single-nucleotide mismatches in DNA and RNA targets through excimer signal formation.
The synthesized 2′-amino-α-L-LNA monomer X with a pyrene moiety provides a tool for detecting single nucleotide polymorphisms in DNA and RNA.
May advance mismatch detection probes; leaves open diagnostic translation pending validation.
Precise positioning of intercalators furnishes a SNP-detection tool. The conformationally locked 2-oxo-5-azabicyclo-[2.2.1]heptane skeleton of 2′-amino-α-L-LNA monomer X directs the N2′-linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single-nucleotide mismatches in DNA/RNA targets by excimer signal formation.
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Kumar et al. (2007) studied this question. 2′-N-(Pyren-1-yl)acetyl-2′-Amino-α-L-LNA probes was evaluated on Detection of single-nucleotide mismatches in DNA/RNA targets by excimer signal formation. 2′-N-(Pyren-1-yl)acetyl-2′-Amino-α-L-LNA probes successfully signaled the presence of single-nucleotide mismatches in DNA and RNA targets through excimer signal formation.
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