Development of Grape Microsatellite Markers and Microsatellite Analysis Including Oriental Cultivars

Nine newly developed microsatellite markers for grapes were characterized. Using these markers and eight reported markers, seven Occidental and eight Oriental cultivars were analyzed, including Japanese and Chinese cultivars of Vitis vinifera, two cultivars of V. labrusca, and one sample each of V. riparia and V. rotundifolia. Calculated phenetic distances (1 − proportion of shared alleles) agreed well with the classification of grapes. A dendrogram based on the phenetic distances showed a clear separation of species of Vitis, as well as Oriental and Occidental cultivars. Results suggested the importance of Oriental cultivars as genetic resources of grapes.