Reproductive tissues from male house and field crickets were investigated for the presence of arachidonic acid. Arachidonic acid was identified by retention behavior on 3 gas liquid chromatographic column systems-SE-30, OV 225, and Silar 10C-and by gas liquid/positive ion chemical ionization mass spectrometry. Arachidonic acid constituted 0.3 and 1.2% of total fatty acids in the house and field cricket, respectively. Freeze-dried male reproductive tissues from house and field cricket contained ca. 0.02 and 0.16% arachidonic acid and 8.2 and 8.6% total fatty acids. Other fatty acids found in reproductive tracts of both species of crickets were 16∶0, 18∶0, 18∶1, 18∶2, 20∶0, 20∶1 and 20∶3.
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Worthington et al. (1981) studied this question.
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