First study of complete genome of Dengue-3 virus from Rajasthan, India: genomic characterization, amino acid variations and phylogenetic analysis

First study on the complete genome characterization of Dengue-3 virus is reported from dengue endemic state Rajasthan, India. The genome of 10,672 base pairs was studied with reference to global and the regional genomes of Dengue-3 virus. 388 variations were observed in the nucleotide sequences with reference to the NCBI genome, including 34 variations in the amino acids. Of these 34 AA variations, 4 variations showed AA substitution in Envelope proteins and 1 in Anchored capsid region. The remaining 29 variations were observed in amino acids constituting non structural (NS) proteins. The reported mutations, especially those leading to amino acid variations in non-structural proteins of virus may influence the clinical profile of patients and accuracy of ongoing diagnostic tests. Reported amino acid variations in virus envelope may influence the immune response of patients. Phylogenetic studies showed similarity of reported genome with genomes from India, Pakistan and China.