Hypermethylation of Chromosome 17P Locust D17S5 in Human Prostate Tissue

No AccessJournal of UrologyInvestigative Urology1 Aug 1996Hypermethylation of Chromosome 17P Locust D17S5 in Human Prostate Tissue Ronald A. Morton, John J. Watkins, Steven G. Bova, Michelle Makos Wales, Stephen B. Baylin, and William B. Isaacs Ronald A. MortonRonald A. Morton , John J. WatkinsJohn J. Watkins , Steven G. BovaSteven G. Bova , Michelle Makos WalesMichelle Makos Wales , Stephen B. BaylinStephen B. Baylin , and William B. IsaacsWilliam B. Isaacs View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65916-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Under normal conditions genomic CpG islands are not methylated. Hypermethylation of a CpG island in the 5' regulatory region of a gene has the capacity to silence gene transcription. Recently, hypermethylation of a CpG island at D17S5 on chromosome 17P13.3 has been shown to be a frequent tumor-specific event. When it has been observed, hypermethylation of D17S5 occurs solely in neoplastic tissues. Consequently, it has been hypothesized that hypermethylation of D17S5 may be an important carcinogenic event in the organs in which it occurs (colon, kidney, and brain). In this study we examine D17S5 hypermethylation in DNA from the prostate, a gland which is unique in that it undergoes hyperplastic or neoplastic growth or both in virtually all aging men. Materials and Methods: The methylation sensitive restriction enzyme Notl, a cDNA probe specific for the D17S5 locus, and Southern blotting were used to assay for hypermethylation of D17S5 in DNA derived from normal, benign hyperplastic and malignant prostate tissues. Results: We find that methylation of Notl restriction sites at D17S5 is a very common occurrence in prostate cancers (25 of 26 cases examined). 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Google Scholar 29 : Cytidine methylation of regulatory sequences near the pi-class glutathione S-transferase gene accompanies human prostatic carcinogenesis. Proc. Natl. Acad. Sci. U.S.A.1994; 91: 11733. Google Scholar James Buchanan Brady Institute of Urology and The Johns Hopkins Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland.Requests for reprints: Scott Department of Urology, Baylor College of Medicine, 6535 Fannin, F421, Houston, Texas 77030.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 156Issue 2August 1996Page: 512-516 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information Ronald A. Morton More articles by this author John J. Watkins More articles by this author Steven G. Bova More articles by this author Michelle Makos Wales More articles by this author Stephen B. Baylin More articles by this author William B. 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