A rapid high-performance liquid chromatography method for the simultaneous estimation of water-soluble vitamin in ten wild edible plants consumed by the tribal people of North-eastern Region in India

cordata, Oenanthe linearis, Potentilla lineata, Perilla ocimoides, Sonchus arvensis, and Zanthoxylum acanthopodium are potent wild edible plants and consumed by the tribal people of North-eastern region in India.Objective: A reversed-phase high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous quantitation of several water-soluble vitamins such as ascorbic acid (Vitamin C), thiamine (Vitamin B 1 ), riboflavin (Vitamin B 2 ), niacin (Vitamin B 3 ), pantothenic acid (Vitamin B 5 ), pyridoxine (Vitamin B 6 ), and folic acid (Vitamin B 9 ) in these ten wild edible plants. Materials and Methods:The chromatographic separation of vitamins was carried out on Acclaim C 18 column (5 µm particle size, 250 mm × 4.6 mm), Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (210, 245, and 254 nm) using a mobile phase of acetonitrile and aqueous trifluoroacetic acid (0.01% v/v) solution with gradient elution.Conclusion: The results of investigation showed that these plants are rich sources of vitamins, especially the B group of vitamins that can contribute immensely to nutrition, food security, and health and therapeutic benefits.The high percentage of recovery (98%-99%), low coefficient of variation (R 2 > 0.99), low limit of detection, and limit of quantitation confirm the suitability of the method for simultaneous quantification of vitamins in these ten plants under investigation.