The addition of phenol (about 1%) to 6m HC1 largely prevented destruction of tryptophan during hydrolysis ofpeptides at 1 10°C for 22hr.Tryptophan recovery depended on the volume of 6mHC1containing phenol and the concentration of phenol.The maximumtryptophan recovery was 85%for a standard amino acid mixture.The recovery was slightly lower for proteins.This hydrolytic procedure was advantageous for micro aminoacid analysis using a conventional highperformance liquid chromatography with a precolumn labeling technique.The method was used in the amino acid sequence analysis of a minor component of sea anemone toxins isolated from Anthopleura fuscoviridis.The toxin consisted of 48 amino acid residues with three tryptophan residues.Tryptophan in peptides and proteins is destroyed during conventional hydrolysis with 6m HC1 at 110°C for 22-24hr.Various meth-
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Muramoto et al. (1987) studied this question.