Clinical Course and Gross Pathological Findings in Wild Boar Infected with a Highly Virulent Strain of African Swine Fever Virus Genotype II

Figures

• 

  Figure 1. Average of clinical scores and body temperatures of wild boar intramuscularly (IM) inoculated with African swine fever virus (ASFV) Arm07 isolate (n = 6; solid line) and in-contact wild boar (n = 11; dashed line).

• 

  Figure 2. Kaplan-Meier curve showing the survival time in days post-inoculation (DPI) of the wild boar intramuscularly (IM) inoculated with ASFV Arm07 strain (n = 6; continues line) and the in-contact wild boar (n = 11; dashed line).

• 

  Figure 3. Guideline of macroscopic figures to evaluate the main pathological findings during the external examination and autopsy of wild boar infected with a highly virulent strain of African swine fever virus genotype II, Armenia 2007. (a) Conjunctiva and eyelids: Score 1—slight congestion; score 2—swelling of eyelids and serosanguineous ocular discharge; score 3—congested ocular mucosa with sclera hemorrhages, inflamed eyelids, and hyphemia. (b) Skin: Score 1—areas with skin erythema (ears, flanks, abdomen); score 2—erythema of the skin, multiple hemorrhages; score 3—erythema of the skin, multifocal to coalescent hemorrhages. Subcutaneous edema. Occasionally necrotic areas. (c) Heart: Score 1—petechial hemorrhages on endocardium; score 2—multifocal hemorrhages on the cardiac muscle and valvular endocardium; score 3—extensive coalescent hemorrhages on the cardiac muscle and valvular endocardium. A variable degree of valvular edema is observed in the atrioventricular valve. (d) Lung: Score 1—Absence of pulmonary parenchyma collapse associated with dilatation of interlobular septa, due to interstitial edema and the presence of some petechiae (interstitial pneumonia with severe pulmonary…

• 

  Figure 4. Guideline of macroscopic figures to evaluate the main pathological findings during the external examination and autopsy of wild boar infected with a highly virulent strain of African swine fever virus genotype II, Armenia 2007. (a) Tonsil: Score 1—erythematous; score 2—swelling and erythematous with fibrin on the surface; score 3—swelling and erythematous with fibrin/pus and multifocal necrosis on the surface. (b) Lymph node: Score 1—moderate color changes with occasional petechiae; score 2—petechiae and ecchymosis; score 3—increased size, edematous and hemorrhagic. Generalized lymphadenomegaly. (c) Stomach: Score 1—acute catarrhal gastritis; score 2—acute catarrhal gastritis with numerous petechiae/ecchymosis on the surface; score 3—hemorrhagic gastritis (more than 50% affected) with occasionally erosive lesions. (d) Intestine: Score 1—acute catarrhal enteritis with some petechiae on serosa surface; score 2—acute catarrhal enteritis with numerous petechiae/ecchymosis on serosa surface; score 3—hemorrhagic enteritis. I Pancreas: Score 1—multifocal hemorrhages on the surface; score 2—focal necrosis; score 3—extensive necrosis.

• 

  Figure 5. Guideline of macroscopic figures to evaluate the main pathological findings during the external examination and autopsy of wild boar infected with a highly virulent strain of African swine fever virus genotype II, Armenia 2007. (a) Liver: Score 1—mild multifocal parenchymatous color changes with slight hepatomegaly; score 2—moderate hepatomegaly, moderate congestion and hemorrhages. Mild diffuse lobular pattern; score 3—intense hepatomegaly, intense congestion and parenchymatous coalescent red zones; moderate panlobular pattern. (b) Kidney: Score 1—minimal to mild multifocal cortical and medullar petechiae; score 2—moderate multifocal cortical and medullar petechiae with a preponderance of cortical affectation; score 3—marked multifocal corticomedullar hemorrhages more frequent on the cortical layer with/without multifocal of moderate dark red pelvic areas. Perirenal edema. (c) Urinary bladder: Score 1—mild multifocal petechiae on mucosa surface with/without mild color changes in the urinary bladder wall; score 2—multifocal petechiae and ecchymosis on urinary bladder wall with edema. Turbid urine; score 3—multifocal to coalescent hemorrhages on the mucosal surface. Intense…