Key result
Aspirin attenuates osteogenic vascular smooth muscle cell calcification and osteoblastic marker expression.
Why the study?
Vascular calcification is a major complication of chronic renal failure driven by osteogenic transition of VSMCs, and aspirin's effect on VSMC calcification is unclear.
Does aspirin reduce calcification in vascular smooth muscle cells?
Does aspirin reduce calcification in vascular smooth muscle cells?
Aspirin alleviates vascular smooth muscle cell calcification in vitro by enhancing the HSP70- and HSP90-mediated heat shock response.
May support aspirin investigation in vascular calcification; leaves open any clinical translation in CKD.
CONTEXT: Vascular calcification is a major complication of chronic renal failure, which has been identified as an active process partly driven by osteogenic transition of vascular smooth muscle cells (VSMCs). Aspirin could prevent cardiomyocyte damage by inducing heat shock response. OBJECTIVE: This study investigates the effect of aspirin on alleviating VSMC calcification. MATERIALS AND METHODS: VSMC calcification model was established by 10-day calcification induction in osteogenic medium. VSMCs were grouped as following: control group (normal medium), calcified group (osteogenic medium) and treated group (osteogenic medium with 1 or 4 mmol/L aspirin). VSMC calcification was evaluated by calcified nodules formation, intracellular calcium concentration and osteoblastic marker (OPN and Runx2) expression. The elevated expression of OPN and Runx2 induced by osteogenic medium was significantly relieved after 1 or 4 mmol/L aspirin treatment. The expression of HSF1, HSP70 and HSP90 was decreased in calcification-induced VSMCs, but significantly increased after treatment of aspirin. Furthermore, inhibition of HSP70 (or HSP90) by small-molecule inhibitor or small interfering RNA could partially abolish the anti-calcification effect of aspirin, proved by the changes of intracellular calcium concentration and osteoblastic marker expression. DISCUSSION AND CONCLUSIONS: Aspirin could relieve the calcification of VSMCs partially through HSP70- or HSP90-mediated heat shock response. These findings expanded the understanding of aspirin pharmacology, and imply that local induction expression of HSPs might be a potential therapeutic strategy for the prevention and therapy of vascular calcification.
No takes yet. Share an insight, caveat, or question.
Shen et al. (2021) studied Vascular calcification. Aspirin vs. Control group (normal medium) and calcified group (osteogenic medium) was evaluated on VSMC calcification (calcified nodules formation, intracellular calcium concentration and osteoblastic marker expression) (p=< 0.01). Aspirin treatment (1 or 4 mmol/L) significantly relieved osteogenic medium-induced vascular smooth muscle cell calcification and osteoblastic marker expression (P<0.01).
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: