Fruit body formation of Boletus reticulatus in pure culture

The ectomycorrhyzal fungus Boletus reticulatus formed young fruit bodies in pure culture on liquid and solid media. Primordia formation started 31–32 d after inoculation on liquid medium. The primordia developed into the young fruit bodies with convex pileus and clavate stipe 44 d after inoculation on liquid medium. The ability of this fungus to form fruit bodies declined at one and half years after isolation. Sufficient nutrient in medium is required for the fungus to form mature fruit bodies in pure culture.