An emulsion overlay plating technique was developed for the rapid and selective isolation of yeast that assimilate water immiscible substrates. The plating medium contains a defined enrichment broth and an Aniline blue agar base. Growth is easily detected by colony dye concentration, decolorization zones around colonies and fluorescence of colonies under long wave UV light.
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Cirigliano et al. (1983) studied this question.
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