Acetone and glycerol are only partially miscible at room temperature. Addition of up to 50 mole‐% water is allowed without removing the miscibility gap. By varying the water content, the polarities of the raffinate and extract phases can be adjusted to favorite conditions for the extraction of proteins. Short settling and coalescence times result from both rather high density differences and a relative high interfacial tension of up to 7 mN/m. Thus, the system can be handled in common extraction columns, which is an important process design aspect. Recovery of proteins from both phases is very simple, as this liquid‐liquid extraction system does not contain polymers of a molecular weight similar to the proteins. Distribution equilibria of peroxidase, alk. phosphatase and oxytocin will be given.
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Hampe et al. (1989) studied this question.
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