Improved Determination of Mebeverine Hydrochloride in Urine, Serum and Pharmaceutical Preparations Utilizing a Modified Carbon Paste Electrode

A simple, rapid and sensitive method for the determination of mebeverine hydrochloride (MBHCl) in urine, serum and pharmaceutical preparations using modified carbon paste electrodes was developed. The electrochemical sensors showed a linear dynamic range of 3.0 × 10-7 – 1.0 × 10-2 and 1.0 × 10-7 – 1.0 × 10-2 mol L-1 with detection limit of 3.0×10-7 and 1×10-7 mol L-1 for modified carbon paste (MCPEs; sensors I and II), respectively. The slopes of the calibration graphs are 56.78±0.85 and 58.80±0.46 mV decade-1 for MCPEs with tricresylphosphate (TCP) (sensor I) and o-nitrophenyloctylether (o-NPOE) (sensor II) as plasticizers, respectively. The response time was relatively quick in the whole concentration range (8 and 7 s), respectively. The electrodes can be used at least 65 and 73 days without observing any deviations in a pH range of 2.0–8.0 and 1.5–8.0 for electrodes I and II, respectively. The fabricated electrodes displayed good selectivity for MBHCl with respect to number of common foreign inorganic cations, sugar species, and glycine as the fillers that may be added to the pharmaceutical preparation. Fortunately, such materials mostly do not interfere. The sensors were successfully applied for the determination of MBHCl in its tablets, urine and serum. The results obtained using these potentiometric electrodes were comparable with those obtained using official method.