Key result
eGFP-tagged retroviral envelope proteins allowed direct visualization of viral particles, efficient infection of cells, and monitoring of virus-cell interaction dynamics.
The development of eGFP-tagged retroviral envelope proteins enables direct visualization and monitoring of virus-cell interactions without indirect labeling.
GFP-tagged Env enables direct retroviral visualization and receptor staining; leaves open broader research applications pending validation.
Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein and efficiently infected cells. We used the GFP-tagged viruses for staining retrovirus receptor-positive cells, thereby circumventing indirect labeling techniques. By generating cells which conditionally expressed the GFP-tagged Env protein, we could confirm an inverse correlation between retroviral Env expression and infectivity (superinfection). eGFP-tagged virus particles are suitable for monitoring the dynamics of virus-cell interactions.
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Spitzer et al. (2003) studied this question. eGFP-tagged retroviral envelope protein was evaluated on Direct visualization of viral particles and virus-cell interactions. eGFP-tagged retroviral envelope proteins allowed direct visualization of viral particles, efficient infection of cells, and monitoring of virus-cell interaction dynamics.
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