The Determination of a Small Amount of Biological Constituent by the Use of Chemiluminescence. V. An Iron-dyestuff Complex as a Catalyst

The catalytic activity of the iron(III) α,β,γ,δ-tetraphenylporphinetrisulfonic acid complex for the chemiluminescence reaction between luminol and H2O2 has been found to decrease in the presence of protein. On the basis of this phenomenon, 2×10−5–2×10−3 g dm−3 bovine serum albumin was determined with the detection limit of 4 ng, lower than that in the previous paper.