Key result
Dasabuvir inhibited human norovirus infection in human intestinal enteroids with EC50 values ranging from 7.55 to 12.41 μM without significant cytotoxicity.
Why the study?
Does dasabuvir inhibit human norovirus infection in human intestinal enteroids?
Does dasabuvir inhibit human norovirus infection in human intestinal enteroids?
Effect estimate: EC50 7.55 to 12.41 μM
Dasabuvir demonstrates antiviral activity against human norovirus, SARS-CoV-2, and rotavirus in human intestinal enteroid models, suggesting potential as a broad-spectrum antiviral agent.
Hypothesis-generating for dasabuvir against norovirus; leaves open need for in vivo and clinical validation before any therapeutic consideration.
Human noroviruses (HuNoVs) are acute viral gastroenteritis pathogens that affect all age groups, yet no approved vaccines and drugs to treat HuNoV infection are available. In this study, with a human intestinal enteroid (HIE) culture system where HuNoVs are able to replicate reproducibly, we screened an antiviral compound library to identify compound(s) showing anti-HuNoV activity. Dasabuvir, which has been developed as an anti-hepatitis C virus agent, was found to inhibit HuNoV infection in HIEs at micromolar concentrations. Dasabuvir also inhibited severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human A rotavirus (RVA) infection in HIEs. To our knowledge, this is the first study to screen an antiviral compound library for HuNoV using HIEs and we successfully identified dasabuvir as a novel anti-HuNoV inhibitor that warrants further investigation.
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Hayashi et al. (2021) studied Human norovirus infection. Dasabuvir vs. DMSO control was evaluated on Reduction in human norovirus RNA genome equivalents (EC50) (EC50 7.55 to 12.41 μM). Dasabuvir inhibited human norovirus infection in human intestinal enteroids with EC50 values ranging from 7.55 to 12.41 μM without significant cytotoxicity.
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