Antigen Synthesis: The Preparation of Selected Dodecapeptide Carriers with Systematically Altered Structures by a Two‐Phase Method

The synthesis of a series of dodecapeptide ethyl esters containing L‐leucine, L‐ala‐nine, glycine and L‐lysine is described. These peptides are suitable carriers for the preparation of haptenic conjugates to be used in antigenicity studies. The strategy for preparing intermediary hexapeptides has been called a two‐phase approach since the elongation of the peptide chain is carried out stepwise in a water‐immiscible organic phase, whereas removal of side‐products and excess reagent is effected by means of a suitably adapted aqueous phase. The method allows an efficient production of relatively large amounts of fully defined peptide antigens.