Development of a novel active bilayer film incorporated postbiotics: investigation of its characteristics and effects on shelf-life extension of chicken breast fillet

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This research aimed to develop and investigate the physical, mechanical, structural, antimicrobial, and antioxidant characteristics of the active bilayer alginate/chitosan film containing postbiotic metabolites of Lactiplantibacillus plantarum A10. Different concentrations of postbiotics (0, 8, 10, 12 and 14 mg/mL) were used in making the bilayer films. Then, the effect of chicken fillet wrapping with this active film on its shelf-life extension at 4 °C was investigated. It was observed that by increasing the postbiotic concentrations up to 14 mg/mL, the inhibition zones against pathogenic indicators Escherichia coli (PTCC 1399) and Listeria monocytogenes (PTCC 1298) were significantly increased to 25 mm compared to the control film (13-15 mm). Also, solubility, moisture content and tensile strength of films were significantly reduced while total color difference, water vapor permeability and elongation at the break were increased. The postbiotic bilayer films revealed more radical scavenging activity (10.94 -20.11%) than control (6.31%). According to total microbial count, wrapped chicken fillets with postbiotic film (14 mg/ml) had 5-days longer shelf-life than the unwrapped sample. The coated chicken fillet obtained a higher overall acceptance value in sensorial analysis. Therefore, chicken fillets packaging with postbiotic bilayer film could successfully suppress microbial proliferation and extend shelf life.