Effect of Incubation and Cold Shock on Motility of Boar Spermatozoa and Their Relationship to Lipid Content

During aerobic incubation of boar spermatozoa for 0 to 5 hr. at 38° C., percent progressive motility decreased markedly from 0 to 1 hr., decreased slower from 1 to 4 hr. and decreased again from 4 to 5 hours. There was no difference (P<.05) in motility of spermatozoa after cold shock in Tris buffer compared to Tris plus 5% EY. Highly significant (P<.01) positive correlations were found between the cold shock treatments and within the incubation times, but not between cold shock and incubation. There were significant positive correlations between motility and dry weight of gel, seminal plasma and spermatozoa and significant negative correlations between the percent lipid in these fractions and motility.