Optimization of acquisition and processing times for the measurement of 1H to 13C polarization transfer kinetics

This is the authors' abstract. We don't add key points for this paper.

Solid-state NMR (ssNMR) is a unique technique allowing the study of macromolecular assemblies in their native state without prior modification. The proposed method simplifies the current long and tedious data-acquisition and processing protocols for Variable Contact Time (VCT) ssNMR experiments used in dynamic studies of macromolecular assemblies. Using cellulose nanocrystals as a model for polysaccharide assembly, the acquisition time was reduced by decreasing the number of scans and shortening the recycling time required for the 1 H to 13 C polarization transfers. •A tenfold reduction in the acquisition time for each kinetic point was achieved while maintaining a good signal to noise ratio. •The processing time for the pseudo-2D solid-state NMR data was also shortened by using signal intensities derived from peak picking rather than the classically-used spectral deconvolution method. •These optimizations enabled the molecular dynamic parameters T 1ρ H , T HH , T CH to be accessed within a day.