Key result
Prostaglandin E2 dose-dependently inhibited COX-2 protein expression and COX activity in IL-1beta-treated human umbilical vein endothelial cells, an effect reversed by forskolin.
Why the study?
Does PGE2 inhibit the induction of COX-2 in IL-1beta-treated human umbilical vein endothelial cells?
Population
Human umbilical vein endothelial cells (HUVEC) treated with interleukin-1beta (IL-1beta 1 ng/ml)
Comparison
Prostaglandin E2 with or without forskolin vs Untreated HUVEC or HUVEC treated with IL-1beta…
Design
Preclinical
Follow-up
24 hours
Authors
Loading...
Supports negative feedback on endothelial COX-2 via cAMP; leaves open in vivo relevance or therapeutic targeting in cardiovascular inflammation.
Does PGE2 inhibit the induction of COX-2 in IL-1beta-treated human umbilical vein endothelial cells?
PGE2 initiates negative feedback regulation of COX-2 induction elicited by IL-1beta in endothelial cells, mediated by cAMP.
Akarasereenont et al. (1999) studied this question. Prostaglandin E2 (PGE2) vs. Untreated HUVEC / IL-1beta treated HUVEC without PGE2 was evaluated on COX-2 protein expression and COX activity. Prostaglandin E2 dose-dependently inhibited COX-2 protein expression and COX activity in IL-1beta-treated human umbilical vein endothelial cells, an effect reversed by forskolin.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: