Chalkley 's method for determination of the nucleocytoplaamic ratio has been employed to study this feature in aging of the liver in the rat.An increase with age occurs, the volume of the nuclei becoming greater relative to that of the cytoplasm.Evidence is presented that the change is due to absolute increase in nuclear volume rather than loss of cytoplasmic volume.There is an increase in numbers of binucleate and multinucleate liver cells in old animals.Giant nuclei and nuclei containing ipdusion bodies are distinctive features of senile liver.The possible nature of the inclusion bodies is discussed.The mitochondria in liver cells of young rats are evenly distributed in the cells.In old rats they are irregular in distribution, some cells having very few, others very many mitochondria1 elements.The Feulgen reaction was used to study the thymonucleic acid content of the nuclei.No marked changes in amount of thymonucleic acid were found.There is little change in hepatic connective tissue with age.Studies on human autopsy material &ow a close correlation between the age changes in rat and in man.1 Supported by 8
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A 1952 study studied this question.