Key result
Heat treatment of red blood cells increased the characteristic times of rouleau formation and slightly reduced the disaggregation shear rate without significantly changing disaggregation shear stress.
Why the study?
Does heat treatment alter the aggregation behavior of human red blood cells?
Does heat treatment alter the aggregation behavior of human red blood cells?
Heat-induced alterations of erythrocytes affect the mechanical properties of the cell membrane without significantly changing macromolecular bridging energy.
Heat effects on RBC aggregation kinetics merit in vivo validation; leaves open relevance to microcirculation in hyperthermia or transfusion.
The aggregation behaviour of normal and heat treated (48.4 degrees C, 48.8 degrees C, 49.5 degrees C) red blood cells (RBCs) suspended in dextran-saline solutions (Dx 70, Dx 173) was investigated by a laser light reflectometric method over a wide range of bridging energies. The characteristic times of rouleau formation were found to be increased after RBC heat treatment. The disaggregation shear stress is not significantly different between normal RBCs and heat treated RBCs. The loss of cell deformability is nevertheless shown to improve slightly the dissociation efficiency of the flowing liquid in a shear flow resulting in a small reduction of the disaggregation shear rate after heat treatment. Heat treatment is also shown to alter the structure of RBC network at equilibrium. These results indicate that heat induced alterations of erythrocytes only affects the mechanical properties of the cell membrane without significant changes in the macromolecular bridging energy.
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Snabre et al. (1985) studied Normal and heat treated red blood cells. Heat treatment (48.4, 48.8, 49.5 degrees C) vs. Normal red blood cells was evaluated on Aggregation behaviour (characteristic times of rouleau formation, disaggregation shear stress, disaggregation shear rate). Heat treatment of red blood cells increased the characteristic times of rouleau formation and slightly reduced the disaggregation shear rate without significantly changing disaggregation shear stress.
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