A new rhodamine fluorescent probe for monitoring ˙OH has been developed based on the oxidative C-H abstraction reaction of rhodamine cyclic hydrazide. The probe exhibits excellent selectivity for ˙OH with virtually no interference by other ROS/RNS species. Fluorescent imaging of A549 and RAW264.7 cells is also successfully demonstrated to detect intracellular ˙OH in live cells.
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Kim et al. (2013) studied this question.
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