Regenerasi dan Pertumbuhan Beberapa Varietas Tebu (Saccharum officinarum L.) secara In Vitro

(Saccharum officinarum L.) through In Vitro Culture. Deden Sukmadjaja and Ade Mulyana. The research was conducted at the Laboratory of Tissue Culture The Biology of Cell and Tissue Researcher Group ICABIOGRAD, Bogor from June to November 2009 to studied growth and regenerations response some varieties of sugarcane through in vitro culture. The research activities have been carried out in three steps, i.e., callus formation, regeneration of shoots and roots regeneration. The type of explants used in the study was in vitro planlet explants of both sugarcane varieties. Seven media formulations were used for the callus induction and regeneration of shoots, while five media formulations were used for the roots regeneration. The results showed that the highest respond for calluses induction was Bulu Lawang varieties at media formulation MS + 2.4-D 2 mg.l-1 + BAP 0.4 mg.l-1 + CH 2000 mg.l-1 and PS 951 varieties at media formulation MS + 2.4-D 1 mg.l-1 + BAP 0.4 mg.l-1. While the highest respond for regeneration of shoots was Bulu Lawang varieties at media formulation MS0 (control MS) dan PS 951 varieties at media formulation MS + BAP 1 mg.l-1 + kinetin 1 mg.l-1 + NAA 0.5 mg.l-1 + GA3 0.5 mg.l-1. The highest respond of roots regeneration was Bulu Lawang and PS 951 varieties at media formulation MS + IBA 1 mg.l-1. Acclimatization of plantlets produced were grew successfully about 90-100% in greenhouse.