Determination of Phenol Content and Antibacterial Activity of Five Medicinal Plants Ethanolic Extracts from North-West of Morocco

Determination of total phenolic content (TPC)The concentration of the phenolic compounds in the plants extracts was determined using the Folin Ciocalteu assay [10], with some modifications.In brief, the extract was diluted to the concentration of 1 mg /ml, and aliquots of 100 μl or a standard solution of gallic acid (20, 40, 60, 80 and 100 mg/l) were mixed with 500 μl of Folin Ciocalteu reagent (previously diluted 10-fold with distilled water) and 400 μl of Na 2 CO 3 (7%).After 40 min of incubation at room temperature