Key result
Angiotensin II drives cardiac hypertrophy and myocardial apoptosis via PINK1/Parkin-mediated autophagy in mice.
Why the study?
The role of autophagy in angiotensin II-induced cardiac hypertrophy in C57BL/6 mice was not fully understood.
Does Angiotensin II induce cardiac hypertrophy via PINK1/Parkin-mediated autophagy in C57BL/6 mice?
RCT (n=10)
randomly assigned
Does Angiotensin II induce cardiac hypertrophy via PINK1/Parkin-mediated autophagy in C57BL/6 mice?
PINK1/PARKIN-mediated autophagy is involved in Angiotensin II-induced cardiac hypertrophy by affecting myocardial apoptosis and mitochondrial function.
Supports PINK1/Parkin autophagy in Ang II mouse hypertrophy; hypothesis-generating for human remodeling therapies.
OBJECTIVE: To study the role of autophagy in angiotensin II-induced cardiac hypertrophy in C57BL/6 mice. METHODS: We randomly assigned 10 C57BL/6 mice into the control and angiotensin II (Ang II) groups (n = 5 in each group). Ang II group mice were injected with Ang II (3 mg/kg/day). Cardiac structure, myocardial pathological changes, mitochondrial structure, autophagosomes, mitochondrial membrane potential (MMP), and myocardial apoptosis were examined. Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1), Parkin, and microtubule-associated protein1A/1B-light chain 3 (LC3) II protein expression levels and mRNA expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were examined. RESULTS: The heart weight/body weight ratio, posterior wall of the left ventricle, myocardial apoptosis (%), relative number of autophagosomes, ANP and BNP mRNA levels, and PINK1, Parkin, and LC3 II protein levels were significantly higher in the Ang II group than in the control group. The MMP and left ventricular ejection fraction were significantly lower in the Ang II group than in the control group. There was disordered arrangement of cardiomyocytes and mitochondria, and obvious mitochondrial swelling, cardiomyocyte hypertrophy, and fibrosis in the Ang II group. CONCLUSION: PINK1/PARKIN-mediated autophagy is involved in Ang II-induced cardiac hypertrophy by affecting myocardial apoptosis and mitochondrial function.
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Fan et al. (2019) conducted an RCT in angiotensin II-induced cardiac hypertrophy (n=10). Angiotensin II vs. control was evaluated on Cardiac structure, myocardial pathological changes, mitochondrial structure, autophagosomes, mitochondrial membrane potential, and myocardial apoptosis. Angiotensin II injection (3 mg/kg/day) in C57BL/6 mice significantly increased cardiac hypertrophy, myocardial apoptosis, and PINK1/Parkin-mediated autophagy compared to control.
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