Synthesis of silver nanoparticles with green tea-extracted reductants: a preliminary study for optimization of the preparation technique

Introduction: The current research article is focused on the optimization of the preparation technique used for the “green” synthesis of silver nanoparticles. Aim: A primary goal of the study was to define and adjust critical variables in the production process so that maximally small, pure, stable in solution, and eligible for future drug conjunction silver nanoparticles would form. Materials and Methods: Silver nanoparticles were obtained by reducing silver nitrate (1 mM and 10 mM solutions) with Camellia sinensis (green tea)-derived catechins or total green tea aqueous extract in varying concentrations and pH. The samples’ visual appearance and short-term physical stability were observed within 7 days. The formation of nanosilver suspensions was established and semi-quantified by UV-Vis spectral analysis. Results and Discussion: The alkalization of the reducing agent (pH 8) before its utilization revealed potentiation in the silver nanoparticle synthesis and stability. Among all formulations, samples obtained with alkalized purified catechin solution in 1 mM of silver nitrate showed maximally sharped absorbance peaks at 417–424 nm, minimal excess of unattached bioactive compounds, and no signs of turbidity for the suggested test period. Conclusion: These samples were considered the most promising and suitable for further investigation, processing, conjunction, and potential therapeutic application.