Biological activity of enzymatic hydrolysates and the membrane ultrafiltration fractions from perilla seed meal protein

The Perilla seed meal (PSM) protein was hydrolyzed with Flavourzyme; the hydrolysate was fractionated by an ultrafiltration and its physiological activity was measured.Peptides with low molecular weights exhibited higher antioxidant activity, except for the Fe 2+ chelating activity, compared to peptides with a high molecular weight.The IC 50 values of the α-amylase inhibitory activity ranged from 727.89 µg/ml to 757.18 µg/ml, the α-glucosidase inhibitory activity was highest in the < 1 kDa fraction.The < 1 kDa fraction exhibited the strongest angiotensin I-converting enzyme inhibitory activity.As a result, the peptides from PSM protein hydrolysates, particularly peptides < 1 kDa, exhibited excellent antioxidant, antidiabetic, and antihypertensive activities and thus were highly likely to be developed as a functional food material.