Preparation of Fluorescent Poly(methyl methacrylate) Beads Hybridized with Y3Al5O12:Ce3+ Nanophosphor for Biological Application

Poly(methyl methacrylate) beads were hybridized with Y 3 Al 5 O 12 :Ce 3+ nanoparticles through electrostatic interactions using the layer-by-layer adsorption technique to prepare fluorescent beads for biological application. The subsequent sequential adsorption of poly(sodium 4-styrenesulfonate) and poly(allylamine hydrochloride) onto the composite beads prevented the detachment of the Y 3 Al 5 O 12 :Ce 3+ nanoparticles. The fluorescent beads were observed as tight dot plots by flowcytometry. Bovine serum albumin can be immobilized on the surface of the composite beads and tagged with a red dye through an antigen–antibody reaction. This result indicates that the composite beads are useful for bead assays such as flowcytometry.