Key result
Peptide (CKGGRAKDC-NH2) coupled with PEG-coated USPIO nanoparticles significantly decreased the T2 relaxation time of brown adipose tissue in mice compared to controls (P<0.001).
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Preclinical BAT imaging feasible in mice; leaves open human translation of targeted USPIO-MRI.
The peptide (CKGGRAKDC-NH2) specifically targets the brown adipose tissue (BAT). Here we applied this peptide coupled with polyethylene glycol (PEG)-coated ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to detect BAT in vivo by magnetic resonance imaging (MRI). The peptide was conjugated with PEG-coated USPIO nanoparticles to obtain targeted USPIO nanoprobes. Then the nanoprobes for BAT were evaluated in mice. T2⁎-weighted images were performed, precontrast and postcontrast USPIO nanoparticles. Finally, histological analyses proved the specific targeting. The specificity of targeted USPIO nanoprobes was observed in mice. The T2⁎ relaxation time of BAT in the targeted group decreased obviously compared to the controls (P<0.001). Prussian blue staining and transmission electron microscope confirmed the specific presence of iron oxide. This study demonstrated that peptide (CKGGRAKDC-NH2) coupled with PEG-coated USPIO nanoparticles could identify BAT noninvasively in vivo with MRI.
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Hu et al. (2018) studied Brown adipose tissue detection. Peptide (CKGGRAKDC-NH2) coupled with PEG-coated USPIO nanoparticles vs. Controls was evaluated on T2 relaxation time of brown adipose tissue (p=<0.001). Peptide (CKGGRAKDC-NH2) coupled with PEG-coated USPIO nanoparticles significantly decreased the T2 relaxation time of brown adipose tissue in mice compared to controls (P<0.001).
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