Bismuth Film Electrode as Sensing Platform for IgE–anti‐IgE Interactions

In this study, a bismuth film was used as a sensing platform for immunoreaction assay which includes the interactions between IgE and anti‐IgE molecules. For preparing this assay, IgE was deposited on a carbon paste electrode via bismuth(III) cations without using any membrane or functional reagent. The immobilized reagents and interaction of anti‐IgE on this formation were monitored by using atomic force microscopy technique and electrochemical impedance spectroscopy. Then some experimental parameters like the effect of IgE amount and the IgE–anti‐IgE interaction time were optimized. Under the optimal experimental conditions, the binding of anti‐IgE was also examined by following current decrease in differential pulse voltammograms of neutral red.