Key result
A time- and cost-effective protocol for extracting proteins from mouse adipose tissue yields approximately 2.0 mg protein from 100 mg white adipose tissue while minimizing lipid contamination.
Describes a cost-effective and time-saving protocol for extracting high yields of protein from mouse adipose tissue by minimizing lipid contamination.
May improve AT protein extraction for obesity research; leaves open optimal protocol selection without direct comparisons.
As obesity becomes a global epidemic, the metabolism research field is increasingly focusing on studying the physiological and pathological roles of adipose tissues (AT). However, extracting proteins from AT is challenging due to abundant fat content of intracellular lipid droplets. Several commercial kits for extraction of AT proteins are available, as are protocols (such as the RELi protocol as well as other protein precipitation protocols). The protocols have been introduced to improve the quality and yield of extractions, but these methods either increase the cost or involve multiple steps. Herein, we describe a detailed protocol for mouse AT protein extractions based on our daily laboratory practice. This protocol requires only very common reagents and instruments, and can be completed in 90-120 min and provides good recovery of total protein content. Thus, this protocol is an economically attractive, time-saving and efficient way to extract proteins from the AT.
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A 2020 study studied this question. Mouse Adipose Tissue Protein Extraction Protocol was evaluated on Protein yield and lipid depletion. A time- and cost-effective protocol for extracting proteins from mouse adipose tissue yields approximately 2.0 mg protein from 100 mg white adipose tissue while minimizing lipid contamination.
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