Cells of Mycoplasma gallisepticum strains A5969 and S6 were studied by electron microscopy after being treated with hypotonic solutions such as 0.01 M and 0.001 M sucrose and deionized water. It was found that hypotonic solutions induced cells to produce filaments. In addition the outer layer of the membrane increased twofold in width and tended to become amorphous. Hypotonic solutions had no effect on the viability of the cells.
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Ruth Bernstein-Ziv (1971) studied this question.