Impdh1 was identified as a key protein promotes diabetic vasculopathy by intervention of vascular endothelial cell pyroptosis

Figures

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  Fig. 1. Single-cell public dataset analysis. Note: A): UMAP plot of cell annotation results by cell type; B): UMAP plots of cell types for the DDC and Chow groups; C): Bubble plot of markers used to annotate cell types; D): Plot of the cell type proportion in the DDC and Chow groups; E): UMAP plot of EC subsets

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  Fig. 2. Single-cell public dataset analysis. Note: A): Distribution of different subsets of cells in the cell trajectory map. ECs_Impdh1+ (red dots) was mainly distributed at the end branches, while ECs_Impdh1- (blue dots) was mainly at the start branches; B): Trajectory distribution map of cells between the Chow and DDC groups. Trajectory analysis of the Chow group revealed that ECs_Impdh1+ (red dots) appears to diverge from ECs_Impdh1- (blue dots); C): Trajectory distribution map of cells between the Chow and DDC groups, displaying the genetic changes in EC subsets during differentiation; D): Receptor- ligand interaction between cells, displaying the signals sent by ECs_Impdh1- and ECs_Impdh1+ to other cells, where the color and the circle size both represent their different correlations.

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  Fig. 3. Silencing Impdh1 can inhibit aortic tissue damage and pyroptosis in mice. Note: * indicated P < 0.05 compared with the Model group, # indicated P < 0.05 compared with the Model + NC group. A): qRT-PCR assay to determine Impdh1 expression in mice; B): IF staining plot to indicate GSDMD, pro-Caspase1, and NLRP3 expression; C-D): H&E and Masson staining after silencing of Impdh1 to demonstrate the pathological features of aorta in mice and relevant statistical results; E-F): ELISA to detect IL-18 and IL-1β levels

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  Fig. 4. Silencing Impdh1 can ameliorate HG-induced EC injury. Note: * indicated P < 0.05 compared with the NG group, # indicated P < 0.05 compared with the HG + NC group. A): qRT-PCR assay to determine Impdh1 expression in ECs; B): CCK-8 assay to detect the cell viability of ECs; C): IF staining plots to indicate the expression of GSDMD, pro-Caspase1, and NLRP3; D) Cell scratch assay to assess the migration ability; E) Tube formation assay; F-G): ELISA to detect IL-18 and IL-1β levels

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  Fig. 5. Identification and functional enrichment analysis of DEGs in db/db and db/m mice. Note: A): Volcano plot of DEGs between the BLCA and Control in TCGA. Red dots represented up-regulated genes, and blue dots represented down-regulated genes; B): Heat map of the expression of DEGs; C-D): Enrichment bubble plots of GO and KEGG pathway of DEGs; E): Correlation map of Impdh1 with pyroptosis-related genes