Search for Sendai 6/94 viral RNA in the antigen-free cell line Cl-C-2 isolated from human multiple sclerosis brain tissue
Key result
Antigen-free MS brain cell lines show no detectable Sendai 6/94 viral RNA.
Why the study?
The presence of persistent Sendai 6/94 viral RNA in the viral antigen-free cell line Cl-C-2 derived from multiple sclerosis brain tissue was uncertain.
Population
Viral antigen-free cell line Cl-C-2 from multiple sclerosis brain tissue
Comparison
Cl-C-2 versus viral antigen-expressing cell lines Cl-E-8 and Cl-F-2
Design
Nucleic acid hybridization using in vitro synthesized cDNA probe
Authors
Discussion
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Member takes
Implication
No Sendai viral RNA in MS-derived cells leaves open viral persistence in pathogenesis; hypothesis-generating for cellular clearance mechanisms.
Structured PICO
P
Population
Viral antigen-free cell line Cl-C-2 obtained from multiple sclerosis brain tissue by cell fusion with CV-1 cells
E
Exposure
Nucleic acid hybridization using in vitro synthesized cDNA probe
C
Comparator
Viral antigen-expressing cell lines Cl-E-8 and Cl-F-2
O
Outcome
Presence of intracellular virus-specific RNA sequences of the persistent Sendai 6/94 virus
The lack of viral RNA in Cl-C-2 cells suggests that cellular defense mechanisms may eliminate the persistent virus.
Cite This Study
Neubert et al. (1983) studied Multiple sclerosis. Nucleic acid hybridization for Sendai 6/94 viral RNA vs. Viral antigen-expressing cell lines Cl-E-8 and Cl-F-2 was evaluated on Presence of intracellular virus-specific RNA sequences. No Sendai 6/94 viral RNA sequences were detected in the viral antigen-free cell line Cl-C-2 isolated from human multiple sclerosis brain tissue, in contrast to the reference cell lines.