Potential of Biosurfactant as an Alternative Biocide to Control Biofilm Associated Biocorrosion

Background and Objective: The aim of this research was to screen biosurfactants that were potential to be used as an alternative biocide for biofilm associated with biocorrosion.Eight type of biosurfactants extracted from indigenous bacterial isolates were investigated for their ability to eradicate or inhibit biofilm growth.Materials and Method: Biofilm assays were performed using broth microdilution method in microtiter plate 96 wells.The parameters used were Minimum Inhibitory Concentration (MIC), Minimum Biofilm Inhibitory Concentration (MBIC) and Minimum Biofilm Eradication Concentration (MBEC).MIC and MBIC were tested on biofilm consortium liquid culture, while MBEC was tested on preformed biofilm.Results: Biosurfactant of isolate F3 exhibited the highest eradication level (68.3%) at 100 µg mLG 1 .The MIC, MBIC 50 , MBIC 90 and MBEC 50 were observed at 50, 25, 25 and 25 µg mLG 1 , respectively.Based on these results of this study, biosurfactants produced by isolate F3 was potential to be an alternative biocide that can eliminate biofilm-forming microbial consortium.Conclusion: This study affirms that biosurfactant is not only useful for enhancing oil recovery in petroleum industry.It is also potential to be an alternative biocide to eliminate biofilm associated with biocorrosion.