Key result
Genome-wide mRNA expression analysis identified 51 significantly differentially expressed mRNAs (45 down-regulated and 6 up-regulated) in the peripheral blood of patients with obsessive-compulsive disorder compared to healthy controls.
Why the study?
Does genome-wide mRNA expression in peripheral blood differ between patients with OCD and healthy controls?
Case-Control (n=60)
No
Does genome-wide mRNA expression in peripheral blood differ between patients with OCD and healthy controls?
Altered genome expression profiles, particularly involving the ribosomal pathway, may be associated with the development of obsessive-compulsive disorder.
Answers are grounded in this paper and cite their sources.
Peripheral mRNA differences in OCD are hypothesis-generating; larger studies needed before any clinical application.
The onset of obsessive-compulsive disorder (OCD) involves the interaction of heritability and environment. The aim of this study is to identify the global messenger RNA (mRNA) expressed in peripheral blood from 30 patients with OCD and 30 paired healthy controls. We generated whole-genome gene expression profiles of peripheral blood mononuclear cells (PBMCs) from all the subjects using microarrays. The expression of the top 10 mRNAs was verified by real-time quantitative PCR (qRT-PCR) analysis. We also performed an enrichment analysis of the gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) annotations of the differentially expressed mRNAs. We identified 51 mRNAs that were significantly differentially expressed between the subjects with OCD and the controls (fold change ≥1.5; false discovery rate <0.05); 45 mRNAs were down-regulated and 6 mRNAs were up-regulated. The qRT-PCR analysis of 10 selected genes showed that they were all up-regulated, which was opposite to the results obtained from the microarrays. The GO and KEGG enrichment analysis showed that ribosomal pathway was the most enriched pathway among the differentially expressed mRNAs. Our findings support the idea that altered genome expression profiles may underlie the development of OCD.
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Song et al. (2018) conducted a case-control in Obsessive-compulsive disorder (OCD) (n=60). Obsessive-compulsive disorder vs. Healthy controls was evaluated on Differentially expressed mRNAs in peripheral blood mononuclear cells (51 differentially expressed mRNAs, p=<0.05). Genome-wide mRNA expression analysis identified 51 significantly differentially expressed mRNAs (45 down-regulated and 6 up-regulated) in the peripheral blood of patients with obsessive-compulsive disorder compared to healthy controls.
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